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Lumonics Inc scanarray 3000 confocal laser scanner
Scanarray 3000 Confocal Laser Scanner, supplied by Lumonics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/laser+scanner+scanarray+3000/confocal+laser+scanner+scanarray+lite/pmc03878590-256-5-11
Average 90 stars, based on 1 article reviews
scanarray 3000 confocal laser scanner - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Plant seed specific promoters
Article Snippet: Prior to this final concentration step, 1/100 of the labeled probe (approximately 2–4 μL) were removed to determine the quality of the labeling reaction by gel electrophoresis followed by analysis of the fluorescent signal from the separated products using a ScanArray® 3000 laser scanner (GSI Lumonics, Watertown).

Article Title: A 3800 GENE MICROARRAY FOR CATTLE FUNCTIONAL GENOMICS: COMPARISON OF GENE EXPRESSION IN SPLEEN, PLACENTA, AND BRAIN
Article Snippet: A cDNA microarray representing approximately 3800 cattle genes was created for functional genomic studies.. The array elements were selected from >7000 cDNA clones identified in a large-scale expressed sequence tag (EST) project that utilized spleen and normalized and subtracted placenta cDNA libraries.. Sequence similarity searches of the 3820 ESTs represented on the array using BLASTN identified 3290 (86.1%) as putative human orthologs, with the remainder consisting of ‘‘novel’’ genes or highly divergent orthologs.

Article Title: Plant seed specific promoters
Article Snippet: Prior to this final concentration step, 1/100 of the labeled probe (approximately 2-4 μL) were removed to determine the quality of the labeling reaction by gel electrophoresis followed by analysis of the fluorescent signal from the separated products using a ScanArray® 3000 laser scanner (GSI Lumonics, Watertown).

Concentration Assay:

Article Title: Microarray Analysis of Developing Arabidopsis Seeds
Article Snippet: The labeled cDNA products were finally transferred to a Centricon 30 filtration column (Millipore, Bedford, MA), washed twice with 2 mL of Tris-EDTA buffer, and then concentrated to a final volume of 10 to 15 μL using a speed vac. .. Prior to this final concentration step, 1/100 of the labeled probe (approximately 2–4 μL) was removed to determine the quality of the labeling reaction by gel electrophoresis, followed by analysis of the fluorescent signal from the separated products using a ScanArray 3000 laser scanner (GSI Lumonics, Watertown, ME). .. Hybridization Probe mixtures in a total volume of 24 μL were mixed with 6 μL of blocking solution (10 μg/μL of yeast tRNA [Sigma] and 10 μg/μL of oligo-dA [Pharmacia]), 6.3 μL of 20× SSC, and 1.2 μL of 10% (w/v) SDS.

Labeling:

Article Title: Microarray Analysis of Developing Arabidopsis Seeds
Article Snippet: The labeled cDNA products were finally transferred to a Centricon 30 filtration column (Millipore, Bedford, MA), washed twice with 2 mL of Tris-EDTA buffer, and then concentrated to a final volume of 10 to 15 μL using a speed vac. .. Prior to this final concentration step, 1/100 of the labeled probe (approximately 2–4 μL) was removed to determine the quality of the labeling reaction by gel electrophoresis, followed by analysis of the fluorescent signal from the separated products using a ScanArray 3000 laser scanner (GSI Lumonics, Watertown, ME). .. Hybridization Probe mixtures in a total volume of 24 μL were mixed with 6 μL of blocking solution (10 μg/μL of yeast tRNA [Sigma] and 10 μg/μL of oligo-dA [Pharmacia]), 6.3 μL of 20× SSC, and 1.2 μL of 10% (w/v) SDS.

Nucleic Acid Electrophoresis:

Article Title: Microarray Analysis of Developing Arabidopsis Seeds
Article Snippet: The labeled cDNA products were finally transferred to a Centricon 30 filtration column (Millipore, Bedford, MA), washed twice with 2 mL of Tris-EDTA buffer, and then concentrated to a final volume of 10 to 15 μL using a speed vac. .. Prior to this final concentration step, 1/100 of the labeled probe (approximately 2–4 μL) was removed to determine the quality of the labeling reaction by gel electrophoresis, followed by analysis of the fluorescent signal from the separated products using a ScanArray 3000 laser scanner (GSI Lumonics, Watertown, ME). .. Hybridization Probe mixtures in a total volume of 24 μL were mixed with 6 μL of blocking solution (10 μg/μL of yeast tRNA [Sigma] and 10 μg/μL of oligo-dA [Pharmacia]), 6.3 μL of 20× SSC, and 1.2 μL of 10% (w/v) SDS.

Hybridization:

Article Title: Differential interaction of Salmonella enterica serovars with lettuce cultivars and plant-microbe factors influencing the colonization efficiency.
Article Snippet: .. Specific hybridization was analyzed using the ScanArray 3000 confocal laser scanner (GSI Lumonics, Kanata, ON, Canada), measuring the fluorescence of each spot for Cy3 and Cy5 and four background areas around each spot. ..

Fluorescence:

Article Title: Differential interaction of Salmonella enterica serovars with lettuce cultivars and plant-microbe factors influencing the colonization efficiency.
Article Snippet: .. Specific hybridization was analyzed using the ScanArray 3000 confocal laser scanner (GSI Lumonics, Kanata, ON, Canada), measuring the fluorescence of each spot for Cy3 and Cy5 and four background areas around each spot. ..



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